DOI:

Inventory reference

ISSN 1812-7231 Klin.inform.telemed. Volume 16, Issue 17, 2021, Pages 00-00

Author(s)

^1,2Klimova O.M., ²Korobov A.M., ^1,2Bychenko K.O., ¹Kordon T.I.

Institution(s)

¹ГУ "Institute of General and Emergency Surgery named after V.T. Zaitsev National Academy of Medical Sciences of Ukraine"

²Kharkiv National University named after V. N. Karazin

Article title

Influence of different wave lengths photoirradiation on the stages of inflammation and stimulation of stem cells proliferation by exosomes in experiment

Abstract (resume)

The antibiotic resistance formation dictates the search for new approaches to the treatment of chronic inflammation. The successful application of photoirradiation in medicine does not yet answer the question of the mechanisms of light action of different wavelengths on the stages of the inflammatory response.

The aim of the work was to study the parameters of immunoresistance after photoirradiation and the effect of stem cell exosomes on experimental models and substrates. The first experimental model included 5 groups of three month old rats. The 1st - intact animals, the 2^nd - animals with LPS-induced inflammation, the 3^rd - animals with inflammation, irradiated with red light (λ = 630 nm), the 4^th - irradiated with green light (λ = 540 nm); the 5^th - irradiated with blue light (λ = 470 nm).

The second model was a culture of blood leukocytes of patients with chronic inflammation. Used various components of the culture medium. Culture A - medium without mitogen, culture B - medium with PHA mitogen, culture C - medium with the addition of exosomes of mesenchymal stem cells.

We used the methods of light microscopy, spectrophotometry and in vitro cell culture.

At the first stage of the inflammatory reaction, irradiation with red light in group 3rd stimulated inflammation due to endocytosis, which characterizes the digestive ability of phagocytes, activated the formation of circulating immune complexes (CIC) and increased lymphocytotoxicity (LCT) in comparison with these indicators in the second group. In group 4^th, irradiation of animals with green light contributed to an increase in the absorption capacity of neutrophils, a decrease in CIC and LCT, which characterizes the completion of alteration and stimulation of regenerative processes in comparison with the dynamics in the previous groups. The effects after exposure to blue light in animals in group 5^th contributed to the completion of the inflammatory process, which was manifested in a decrease in LCT. The use of exosomes in C cell culture increased proliferative activity by 30%.

Thus, the combined use of a physical factor - photoirradiation using different wavelengths, and a biological factor - exosomes containing metabolites of stem cells, contributed to a reduction in the duration of the stages of the inflammatory process and stimulation of regeneration. In group 4^th, green light contributed to an increase in the absorption capacity of neutrophils, a decrease in CIC and LCT, which characterizes a decrease in alteration and stimulation of regenerative processes.

Keywords

photoirradiation, exosomes, stem cells, inflammation, regeneration

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